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Protino GST/4B Columns

Protino® Glutathione Agarose 4B, best choice for cost-effective GST-tag protein purification

Brand: Macherey-Nagel

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Product CodeProduct Product Description Price (Excl gst) Qty Add
MN745515.1 Protino GST/4B Column 5 mL (1) Protino GST/4B Column 5 mL (1) Login for price  
MN745510.5 Protino GST/4B Columns 1 mL (5) Protino GST/4B Columns 1 mL (5) Login for price  
MN745515.5 Protino GST/4B Columns 5 mL (5) Protino GST/4B Columns 5 mL (5) Login for price  

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Features

  • Highest performance equivalent to Glutathione Sepharose™ 4B / GSTrap™ 4B columns
  • Simply replace your current products, no optimization or protocol change necessary
  • Suitable for small proteins, large protein complexes, proteins with low expression rates universal use
  • Market-leading performance for an unbeatable price
  • Ready-to-use FPLC™ columns filled with Protino® Glutathione Agarose 4B

Product

Pack of

Specification

REF

Protino GST/4B Columns 1 mL

5

FPLCTM columns

MN745510.5

Protino GST/4B Columns 5 mL

1

FPLCTM columns

MN745515.1

 

5

as above

MN745515.5

 

Protino® GST/4B 1 mL

Protino® GST/4B 5 mL

Technology

Affinity chromatography

Affinity chromatography

Chelating ligand

Glutathione, linked via sulfur atom

Glutathione, linked via sulfur atom

Format

1 mL FPLC™ columns

5 mL FPLC™ columns

Column dimensions

0.7 cm (inner diameter) x 2.5 cm (height)

1.6 cm (inner diameter) x 2.5 cm (height)

Column body material

Polypropylen

Polypropylen

Column inlet port

10-32 (1/16'') female

10-32 (1/16'') female

Column outlet port

10-32 (1/16'') male

10-32 (1/16'') male

Matrix

4 % beaded agarose

4 % beaded agarose

Bead size

90 µm

90 µm

Binding capacity*

 10 mg

50 mg 

Max. linear flow rate

250 cm/h

250 cm/h

Max. recommended flow rate

4 mL/min

10 mL/min

Chemical stability

Protino® Gutathione Agarose 4B withstands incubation in 0.1 M acetate 
pH 4, 0.1 M NaOH, 70 % ethanol, or 6 M guanidine hydrochloride without significant loss of protein yield

Storage temperature

4–8 °C

4–8 °C

* Binding capacity will vary for each GST-tagged protein