Protino 96 Ni-NTA | at Mediray

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Protino 96 Ni-NTA

High-throughput purification of His-tag proteins

Brand: Macherey-Nagel

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Product CodeProduct	Product Description	Price (Excl gst)	Qty	Add
MN745425.1 Protino 96 Ni-NTA (1×96)	Protino 96 Ni-NTA (1×96)	Login for price
MN745425.4 Protino 96 Ni-NTA (4×96)	Protino 96 Ni-NTA (4×96)	Login for price

Features

Efficient protein purification using chelating group NTA (nitrilotriacetic acid)
Unique Protino® Purification Plate for leak-free incubation during all steps
Plate outlet and MN Wash Plate prevent cross-contamination
High well-to-well reproducibility
High capacity and high affinity
Purification under native and denaturing conditions
Processing under vacuum or by centrifugation

Protino 96 Ni-NTA includes: Protino® Ni-NTA Agarose, Protino® Purification Plate, MN Wash Plate

Technology	IMAC (immobilized metal ion affinity chromatography)
Chelating ligand	NTA (nitrilotriacetic acid)
Format	96-well plates
Matrix	6 % beaded agarose (cross-linked), precharged with Ni2+
Bead size	45–165 µm
Sample volume	< 750 µL/well, supplied matrix is sufficient for 96 preps with 50 µL of settled agarose beads per well
Bed volume	Variable (50 µL recommended)
Reproducability between wells	± 5 % in yield
Binding capacity*	2 mg per well when using 50 µL of settled agarose
Storage temperature	4–8 °C
* Binding capacity will vary for each polyhistidine-tagged protein and strongly depends on chromatographic conditions