NucleoBond Xtra EF

The new generation of anion exchanger – ultra-fast, endotoxin-free plasmid DNA with high yield

Select your options below

Product CodeProduct	Product Description	Price (Excl gst)
MN740420.10 NucleoBond Xtra Midi EF (10)	NucleoBond Xtra Midi EF (10)	Login for price
MN740420.50 NucleoBond Xtra Midi EF (50)	NucleoBond Xtra Midi EF (50)	Login for price
MN740422.10 NucleoBond Xtra Midi Plus EF (10)	NucleoBond Xtra Midi Plus EF (10)	Login for price
MN740422.50 NucleoBond Xtra Midi Plus EF (50)	NucleoBond Xtra Midi Plus EF (50)	Login for price
MN740424.10 NucleoBond Xtra Maxi EF (10)	NucleoBond Xtra Maxi EF (10)	Login for price
MN740424.50 NucleoBond Xtra Maxi EF (50)	NucleoBond Xtra Maxi EF (50)	Login for price
MN740426.10 NucleoBond Xtra Maxi Plus EF (10)	NucleoBond Xtra Maxi Plus EF (10)	Login for price
MN740426.50 NucleoBond Xtra Maxi Plus EF (50)	NucleoBond Xtra Maxi Plus EF (50)	Login for price

Features

Plasmid DNA with less than 0.05 EU/μg for transfection of highly sensitive cells
Patented endotoxin removal by additional washing step – no extra ice incubation necessary
Optimized column design – Midi prep in approximately 45 min, Maxi prep in 50 min
Column filter included – high filter flow-rates, parallel lysate clearing and loading onto the column
Improved silica material – typical yields of 400 μg (Midi) and 1000 μg (Maxi)
NucleoBond® Xtra Plus EF kits contain NucleoBond® Finalizers to speed up DNA precipitation

Product	Preps	Specification	REF
NucleoBond® Xtra Midi EF kits
NucleoBond® Xtra Midi EF	10	NucleoBond® Xtra Midi Columns with inserted NucleoBond® Xtra Column Filters, buffers, RNase A	MN740420.10
	50	as above	MN740420.50
NucleoBond® Xtra Midi Plus EF	10	NucleoBond® Xtra Midi Columns with inserted NucleoBond® Xtra Column Filters, NucleoBond® Finalizer, buffers, RNase A	MN740422.10
	50	as above	MN740422.50
NucleoBond® Xtra® Maxi EF kits
NucleoBond® Xtra Maxi EF	10	NucleoBond® Xtra Maxi Columns with inserted NucleoBond® Xtra Column Filters, buffers, RNase A	MN740424.10
	50	as above	MN740424.50
NucleoBond® Xtra Maxi Plus EF	10	NucleoBond® Xtra Maxi Columns with inserted NucleoBond® Xtra Column Filters, NucleoBond® Finalizer Large, buffers, RNase A	MN740426.10
	50	as above	MN740426.50

	NucleoBond® Xtra Midi EF		NucleoBond® Xtra Maxi EF
Technology	Anion-exchange chromatography
Format	Midi gravity flow columns		Maxi gravity flow columns
Lysate clarification	Column filters		Column filters
Sample material	< 200 mL (high copy) < 400 mL (low copy)		< 600 mL (high copy) < 1200 mL (low copy)
Vector size	< 300 kbp		< 300 kbp
Typical yield	400 µg		1000 µg
A260/A280	1.8–1.95		1.8–1.95
Endotoxin level	< 0.05 EU/µg DNA		< 0.05 EU/µg DNA
Isopropanol precipitation	Xtra Midi EF Centrifugation	Xtra Midi Plus EF NucleoBond®Finalizer	Xtra Maxi EF Centrifugation	Xtra Maxi Plus EF NucleoBond®Finalizer Large
Preparation time	85 min/prep	45 min/prep	90 min/prep	50 min/prep

Plasmid DNA Kits