1st generation anion exchanger for purification of plasmid DNA
| Application |
High and low copy plasmid DNA purification |
| Target |
Plasmid DNA |
| CE certified |
No, research use only |
| Technology |
Anion exchange chromatography |
| Brand |
NucleoBond |
| Format |
Giga prep |
| Package unit |
5 Prep(s) |
| Handling |
Gravity flow |
| Lysate clarification |
Folded filter |
| Automated use |
No |
| Sample material |
Bacteria, E. coli |
| Sample amount |
500−2000 mL (high copy), 1−4 L mL (low copy) |
| Vector size |
< 300 kbp |
| Typical yield |
2000−10000 µg |
| Theoretical binding capacity |
10000 µg |
| Typical purity A260/A280 |
1.8−1.95 |
| Endotoxin level |
1−10 EU/µg DNA |
| Isopropanol precipitation |
Centrifugation, NucleoBond Finalizer, NucleoSnap Finisher Maxi, NucleoSpin Finisher Midi |
| Preparation time |
120−150 min/2 preps |
| Typical downstream application |
Cloning, PCR, Restriction analysis, Sequencing, Transfection of cells |
| Storage temperature |
18−25 °C |
| Shelf life |
39 Months |
| Scope of delivery |
NucleoBond AX 10000 Columns, NucleoBond Folded Filters (Type 1 and 2), buffers, RNase A |
| Hazardous material |
Yes |
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