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NucleoSpin miRNA

Parallel isolation of small and large RNA

Brand: Macherey-Nagel

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Product CodeProduct Product Description Price (Excl gst) Qty Add
MN740971.10 NucleoSpin miRNA (10) NucleoSpin miRNA (10) Login for price  
MN740971.50 NucleoSpin miRNA (50) NucleoSpin miRNA (50) Login for price  
MN740971.250 NucleoSpin miRNA (250) NucleoSpin miRNA (250) Login for price  
MN740304 NucleoSpin® miRNA Column/Buffer Set NucleoSpin® miRNA Column/Buffer Set Login for price  

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Features

  • RNA purification fractionated by size:
    Isolation of small RNA only (< 200 b),
    Isolation of small RNA (< 200 b) and large RNA (> 200 b) in two separate fractions
    Isolation of total RNA (small and large RNA in one fraction)
  • Additional isolation of total protein fraction ready to use for SDS-PAGE and Western blot analysis
  • Excellent RNA recovery and purity by chaotropic salt lysis without phenol/chloroform
    (patent pending)
  • NucleoSpin® Filters for efficient sample homogenization
  • rDNase for efficient on-column removal of genomic DNA

Product

Preps

Specification

REF

NucleoSpin® miRNA

10*

NucleoSpin® RNA Columns, NucleoSpin® miRNA Columns, NucleoSpin® Protein Removal Columns, Collection Tubes (2 mL, 2 mL lid, 1.5 mL), NucleoSpin® Filters, buffers, RNase-free rDNase

MN740971.10

 

50*

as above

MN740971.50

 

250*

as above

MN740971.250

* Kits for 10/50/250 preps are sufficient for 10/50/250 preps of small and large RNA when isolated in one fraction and are sufficient for 5/25/125 preps of small and large RNA when isolated separately.

Product accessories Pack of Specification REF
NucleoSpin®miRNA Column/Buffer Set 1 set NucleoSpin® RNA Columns (50), Buffer MW1, Buffer MW2 MN740304

Technology

Silica-membrane technology

Format

Mini spin columns

Sample material 

< 107 cultured cells, < 30 mg human / animal tissue
< 50 mg plant tissue, < 150 µL reaction mixture

Fragment size

Small RNA: < 200 nt, large RNA: > 200 nt

Typical yield

10 µg small RNA, 95 µg large RNA (107 HeLa cells)

Elution volume

30–100 µL

Preparation time

< 45 min/6 preps (small and large RNA)
< 35 min/6 preps (small RNA only)

Binding capacity

200 µg