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NEW generation of plasmid midi preparation
Brand: Macherey-Nagel
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NucleoSnap DNA Plasma
Product Code: MN740300.10
NucleoSnap Finisher Midi / Maxi
Product Code: MN740435.10
NucleoVac 24 Vacuum Manifold - MN740299
Product Code: MN740299
API Reagents
Product Code: OT-20120
Features
Product
Preps
Specification
REF
NucleoSnap® Plasmid Midi
10
NucleoSnap® Plasmid Columns, NucleoSpin Plasmid Filter Columns, buffers, RNase A
MN740494.10
50
as above
MN740494.50
Technology
Silica-membrane technology
Processing
Vacuum processing (e.g., using NucleoVac 24 Vacuum Manifold),centrifugation for elution
Format
Snap-off column
Endotoxin level
< 50 EU/µg DNA (EU = Endotoxin Units)
Sample material
Up to 50 mL E. coli culture (OD600=5)
Lysate clarification
NucleoSpin® Plasmid Filter Columns (2 min centrifugation)
Vector size
≤ 25 kbp
Typical yield
400–700 µg (50 mL culture, OD600=4, high-copy plasmid)
A260/A280
1.8–1.9
Elution volume
200–500 µL
Preparation time
35 min/6 preps
Binding capacity
1.5 mg
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