DNA and RNA Kits 

Features

• Simple kit—easy to use
• SeqAmp DNA Polymerase provides robust PCR performance
• In-Fusion HD technology enables fast, easy cloning of RACE fragments
• Requires Only 10 ng of total RNA
• Specific enrichment for either 5' or 3' ends
• No RNA pretreatment required

The SMARTer RACE 5’/3’ Kit is an improved version of the SMARTer RACE cDNA Amplification Kit. The SMARTer RACE 5’/3’ Kit provides improved sensitivity, less background and higher specificity over the previous generation of kits. The first-strand cDNA generated with this kit can be used directly in 5’- and 3’-RACE PCR reactions. The SMARTer protocol does not involve any of the adaptor ligation steps that other RACE kits incorporate, making the protocol shorter and significantly easier to use. The new kit is also designed to accommodate larger RNA input volumes (up to 11 µl) and has been shown to perform more efficiently on challenging targets (e.g., those that are long, GC-rich, etc.). The kit includes our highly robust SeqAmp DNA Polymerase for amplification of products, and the In-Fusion HD Cloning Kit for efficient cloning of RACE fragments in the provided linearized vector. The SMARTer RACE 5’/3’ procedure combines SMART first-strand cDNA synthesis technology with a powerful suppression PCR protocol that vastly reduces the background amplification commonly associated with RACE protocols.