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  5. Nucleic Acid Extraction
  6. DNA and RNA Kits
  7. SMART-Seq v4 3’ DE Kit

SMART-Seq v4 3’ DE Kit

Single-cell transcriptomics is important in understanding how gene expression of individual cells varies within cell populations. We have developed an end-capture method that focuses the majority of sequencing data on the 3' ends of mRNA transcripts from single cells. This enables you to collect high quality data with fewer sequencing reads. This kit incorporates features from the industry-leading SMART-Seq v4 kit, including SMART (Switching Mechanism at 5' End of RNA Template) and locked nucleic acid (LNA) technology.

Brand: Takara

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Product CodeProduct Product Description Price (Excl gst) Qty Add
CLT635040 SMART-Seq® v4 3’ DE Kit, 96 Rxns SMART-Seq® v4 3’ DE Kit, 96 Rxns Login for price  
CLT635041 SMART-Seq® v4 3’ DE Kit, 192 Rxns SMART-Seq® v4 3’ DE Kit, 192 Rxns Login for price  

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The SMART-Seq v4 3' DE Kit includes the components to generate high-quality cDNA from 1-100 intact cells or ultralow amounts of total RNA. The cDNA generated from each reaction has a cell barcode that allows for pooling downstream. This kit uses the SMART-Seq v4 technology with locked nucleic acids (LNA) for excellent sensitivity and reproducibility. Additionally, this kit includes a complete set of index primers for downstream library preparation. By focusing the majority of cDNA fragments on the 3' end of mRNA transcripts, this kit enables differential expression (DE) analysis with fewer reads, reducing the cost of discovery. When combined with the Illumina® Nextera® XT DNA Library Preparation Kit, this kit results in a library ready for sequencing on Illumina platforms.