DNA and RNA Kits 

Applications

• Extraction of viral RNA and DNA from veterinary samples
• Veterinary testing applications

Features

• Small elution volumes for highly concentrated RNA and DNA for maximal sensitivity

Specifications

• Technology: Magnetic bead technology
• Processing: Manual or automated
• Sample material: Whole blood / serum / plasma (< 200 μL), tissue (10–30 mg), feces (< 200 μL), swab wash solution (< 200 μL)
• Maximum amount of starting material: 200 μL liquid / homogenized sample
• Fragment size: 100 bp–approx. 50 kbp
• Elution volume: 50–100 μL
• Binding capacity: 0.4 μg/μL beads
• Processing time: 45–120 min/96 preps (45 min for KingFisher® Flex)

Sample Material

Viruses:

Infectious Bronchitis Virus (IBV), Porcine Circovirus type 2 (PCV-2), Porcine Epidemic Diarrhea Virus (PEDV), Porcine Deltacoronavirus (PDCoV), Porcine Reproductive and Respiratory Virus (PRRSV), Infectious Bursal Disease Virus (IBDV), Bluetongue virus (BTV), Classical Swine Fever virus (CSFV), African swine fever virus (ASFV), Schmallenberg virus (SBV), Avian Influenza Viruses (AIV), Sindbis virus (SINV), Usutu virus (USUV), Batai virus (BATV), Cowpox Virus (CPXV), Giant squirrel respirovirus (GSqRV), Influence D and C Virus (IDV/ICV), Deformed wing virus (DWV), Varroa destructor virus 1 (VDV 1), Acute bee paralysis virus (ABPV), Sacbrood virus (SBV), Israeli acute paral‑ ysis virus (IAPV), Black queen cell virus (BQCV), Chronic bee paralysis virus (CBPV), Kashmir bee virus (KBV)

Bacteria:

Paenibacillus larvae, Melissococcus plutonius, Ascophaera apis, Aspergillus spp., Nosema ceranae, N. apis, Mycoplasma gallisepticum / synoviae

Leading performance in detection of BTV (RNA virus) and PCV 2 (ssDNA virus) after nucleic acid isolation

Viral RNA and DNA was isolated from veterinary samples by using the NucleoMag® VET kit and different competitor kits. Specific qPCR was performed to deter‑ mine the viral titer load in the different sample materials. Detection of BTV (Bluetongue virus) in cattle blood (left). Detection of PCV 2 (Porcine Circovirus Type 2) in pig tissue (right). The NucleoMag® VET kit shows a leading performance among all extraction kits tested.

Data was kindly provided by Dr. Hoffmann, Friedrich-Loeffler-Institut, Germany

Sensitive detection of viral RNA and DNA from animal samples

(A) For purifications, serum samples from PRRSV (blue) and PCV2 (red) positive animal samples were diluted 10-fold. Viral RNA (PRRSV) or viral DNA (PVC2) were isolated from 200 μL blood serum sample using NucleoMag® VET kit. Purified RNA (PRRSV) was analyzed using Real-time Multiplex RT-PCR (VIROTYPE PRRSV, LDL). Purified DNA (PVC2) was analyzed using Real-time PCR (PCV2 Primer). Data was generated in cooperation IVD GmbH Hanover, Germany. (B) PCV2 positive animal tissue samples (n = 22, 20 mg each) were used for viral DNA purification using indicated kits. Purified viral DNA was analyzed by Real-Time PCR (PCV2 Primer). Out of 22 samples 20 (MN) or 14 (Q) samples were tested positive with Ct mean values shown in the graph.

Data kindly provided by Italian customer.