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  5. Nucleic Acid Extraction
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  7. NucleoBond HMW DNA, 20 preps

NucleoBond HMW DNA, 20 preps

MN740160.20

Large amounts of ultrapure, high molecular weight DNA fragments from diverse samples

Brand: Macherey-Nagel

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Features

  • High quality, high molecular weight (HMW) DNA up to 200 kbp
  • Minimized DNA shearing due to established anion exchange technology
  • Validated with diverse samples and sequencing platforms

Contains: NucleoBond® HMW Columns with inserted column filters, plastic washers, buffers, Liquid Proteinase K, Liquid RNase 

Technology Anion exchange chromatography
Format Midi gravity flow columns
Fragment size Up to 200 kbp with > 90% depletion of fragments < 10 kpb
Sample material < 1.5 g plant leaves (ground under liquid nitrogen)
< 107 cultured cells (enzymatic lysis)
< 300 mg solid tissue (cut into small pieces and lysed enzymatically 
   with increased lysis time, ground under liquid nitrogen, or lysed by bead beating)
< 30 mg yeast or bacteria (ground under liquid nitrogen)
< 300 mg yeast or bacteria (lysed enzymatically or by bead beating)
< 2 mL liquid sample (e.g., blood, body fluids or enzymatic reactions)

Typical yield

 Depending on the sample amount and type
Elution volume 50–250 µL
Processing time 2h/12 preps (including 30 min lysis)