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Ribonuclease H, 1,000 Units - TK2150A

TK2150A

Ribonuclease H (RNase H) is an RNA-specific endonuclease that hydrolyzes single-stranded RNA via a magnesium or manganese cofactor. As such, RNase H is often used to remove the RNA template following reverse transcription or cDNA generation. Ribonuclease H (RNase H) is supplied in a buffer of 25 mM Tris-HCl (pH 7.5), 30 mM NaCl, 0.5 mM EDTA, 1 mM DTT and 50% glycerol.

Brand: Takara

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Product CodeProduct Product Description Price (Excl gst) Qty Add
TK2150A Ribonuclease H, 1,000 Units Ribonuclease H, 1,000 Units Login for price  

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Applications

  • Removal of RNA template following second-strand cDNA synthesis
  • Removal of mRNA poly(A) sequence
  • Reverse transcription

Source

Recombinant E. coli

Storage

–20°C

Unit Definition

One unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble product in 20 minutes at 30°C and pH 7.7 using poly(rA)-poly(dT) as a substrate.