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The AAVpro CRISPR/Cas9 Helper Free System (AAV2) is a complete system for delivery of genes encoding the components necessary for CRISPR/Cas9-mediated genome editing (i.e., sgRNA and Cas9 nuclease) to mammalian cells. The included AAVpro CRISPR/Cas9 Vector Set consists of two plasmids (pAAV-Guide-it-Up and pAAV-Guide-itDown) that each contain a truncated, upstream or downstream portion of the Cas9 gene with a 1.6-kb region of homology.
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