ELISA Kits 

Interferon alpha-2 (IFN-α2) is a cytokine encoded by the IFNA2 gene located on chromosome 9. It belongs to the Type 1 IFN family which includes 13 α subtypes, 1 β, and 1 ω. IFN-α2 was the first of these subtypes to be produced pharmaceutically and is the most widely studied. The mature secreted protein consists of 165 amino acids with 5 α-helices and two disulfide bonds. There are a several allelic variants of IFN-α2, including IFN-α2a, IFN-α2b, and IFN-α2c. IFN-α2 can act on all cell types due to the ubiquitous expression of the Type 1 IFN receptor (IFNAR).

IFN-α2 acts to combat infection by stimulating the production of proteins that inhibit viral replication and prevent viral entry into neighboring cells. IFN-α2 can stimulate the immune system by initiating T and NK cell mediated cytotoxicity. IFN-α2 also can inhibit cancer cell proliferation and activate immune system to target cancer cells. IFN-α2 has been used therapeutically in a variety of viral infections (including hepatitis B and C) and certain cancers (such as melanoma, renal cell carcinoma).

The BioLegend Human IFN-α2 ELISA MAX™ Deluxe Set is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The human IFN-α2 capture antibody is first coated on a 96-well plate. Standards and samples are added to the wells and free IFN-α2 binds to the immobilized capture antibody. Next, biotinylated anti-human IFN-α2 detection antibody is added, producing an antibody-antigen-antibody “sandwich”. Avidin horseradish peroxidase is subsequently added, followed by TMB Substrate Solution, producing a blue color in proportion to the concentration of IFN-α2 present in the sample. Finally, the Stop Solution changes the reaction color from blue to yellow, and the microwell absorbance is read at 450 nm with a microplate reader. Different IFN-α2 allelic variants can be detected equally.

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