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Mouse splenocytes or other single cell suspensions are either selected or depleted in a two-step separation process. The cells are first incubated with a BioLegend anti-mouse APC-conjugated antibody, followed by incubating with directly conjugated anti-APC Nanobeads. The APC+, magnetically labeled fraction is retained by the use of a magnetic separator. After collection or depletion of the APC+ cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.
Brand: Biolegend
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