Kit Contents
For BIO480059:
- 200 µl of of Biotin-Antibody Cocktail: Biotin anti- CD3, CD7, CD15, CD19, CD20, CD56, CD57, CD123, CD235ab
- 200 µl Streptavidin Nanobeads
- 1 vial containing 100 µl of Human TruStain FcX™ (Fc Receptor Blocking Solution)
For BIO480060:
- 2 vials of 1 ml of Biotin-Antibody Cocktail each: Biotin anti- CD3, CD7, CD15, CD19, CD20, CD56, CD57, CD123, CD235ab
- 2 vials of 1 ml Streptavidin Nanobeads each
- 1 vial containing 1 ml of Human TruStain FcX™ (Fc Receptor Blocking Solution)
Reactivity Human
Formulation Cocktail: Phosphate buffer solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide.
Preparation The antibodies were purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Strepavidin Nanobeads: Streptavidin-coated magnetic beads.
Storage & Handling
Antibody cocktail, Streptavidin Nanobeads, and Human TruStain FcX™ (Fc Receptor Blocking Solution) should be stored undiluted between 2°C and 8°C.
Application Cell Separation (MojoSort™) - Quality tested
Recommended Usage
10 µl of antibody cocktail for 1 x 107 cells in 100 µl of buffer.
10 µl of Streptavidin Nanobeads for 1 x 107 cells in 100 µl of buffer.
5 µl of Human TruStain FcX™ (Fc Receptor Blocking Solution) for 1 x 107 cells in 100 µl of buffer.
Application Notes
This kit is designed for the isolation of untouched pan monocytes from peripheral blood mononuclear cells (PBMCs).