Kit Contents
For BIO480047:
- 1 vial containing 100 µL of Biotin-Antibody Cocktail: Biotin anti- CD3, CD7, CD15, CD16, CD19, CD20, CD56, CD123, CD235ab
- 1 vial containing 100 µL of Streptavidin Nanobeads
- 1 vial containing 50 µL of Human TruStain FcX™ (Fc Receptor Blocking Solution)
For BIO480048:
- 1 vial containing 1 mL of Biotin-Antibody Cocktail: Biotin anti- CD3, CD7, CD15, CD16, CD19, CD20, CD56, CD123, CD235ab
- 1 vial containing 1 mL of Streptavidin Nanobeads
- 1 vial containing 500 µL of Human TruStain FcX™ (Fc Receptor Blocking Solution)
Reactivity Human
Formulation Cocktail: Phophate buffer solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide.
Preparation The antibodies were purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin. Streptavidin Nanobeads: Streptavidin-coated magnetic beads.
Storage & Handling
Antibody cocktail, Streptavidin Nanobeads, and Human TruStain FcX™ (Fc Receptor Blocking Solution) should be stored undiluted between 2°C and 8°C.
Application Cell Separation (MojoSort™) - Quality tested
Recommended Usage
10 µl of antibody cocktail for 1 x 107 cells in 100 µl of buffer.
10 µl of Streptavidin Nanobeads for 1 x 107 cells in 100 µl of buffer.
5 µl of Human TruStain FcX™ (Fc Receptor Blocking Solution) for 1 x 107 cells in 100 µl of buffer.
Application Notes
This kit is designed for the isolation of untouched CD14+CD16- monocytes from peripheral blood mononuclear cells (PBMCs).
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