The AccuBlue™ High Sensitivity dsDNA Quantitation Kit offers sensitive and selective detection of purified dsDNA samples with minimal effects from common contaminants. The assay provides with a sufficient reagents to perform 1000 assays based on a 96-well microplate format. AccuBlue™ High Sensitivity Quantitation Solution also is available with pre-diluted DNA standards (31006).
The assay is compatible with microplate readers, spectrofluorometers and hand-held minifluorometers. Note that while the assay is spectrally compatible with Qubit® fluorometers from Thermo Fisher, it may not be compatible with the high sensitivity dsDNA program on all Qubit® models. For the Qubit® we recommend our AccuGreen™ High Sensitivity Kit (cat. no. 31066), which was specially formulated for that instrument.
Features
- linear detection range from 0.2 – 100 ng
- λEx/λEm 485/530 nm
- Safer than PicoGreen® or Quant-iT® dsDNA detection kits: the high sensitivity reagent is impermeable to cell membranes
Kit sizes
- The 1000 assay size kit includes AccuBlue™ High Sensitivity Quantitation Solution components and a set of 8 pre-diluted calf thymus dsDNA standards.
- The trial size kit includes one DNA standard solution with instructions for preparing a set of standards by serial dilution.