Overview
- Reproducible results from single cells (or 6–50 pg of DNA)
- Superior reproducibility of allele representation
- Easy to use and automate
- Unambiguous results at all resolutions (e.g., microarray and PCR)
Applications
- CNV analysis using NGS, oligonucleotide aCGH, BAC aCGH, and qPCR
- Target enrichment or amplicon enrichment for mutation detection
- SNP genotyping
Suitable Inputs
- Mammalian cells (1–10 cells)
- Bacterial cells (1,000–10,000 cells)
- Isolated DNA (6–50 pg of human DNA)
- Sorted chromosomes
- Intact or fragmented, single- or double-stranded DNA
The PicoPLEX Single Cell WGA Kit uses a single-tube protocol developed specifically for reproducible amplification of single-copy genomic DNA (gDNA) starting with 1–10 cells or equivalent picogram quantities of isolated gDNA. Cell lysis and whole-genome preamplification are followed by amplification, which results in libraries with very low background and typical yields of 8–12 µg of product in under 3 hours. This system is best suited for applications in which high yield is most important. This product contains reagents for 24 reactions.
The PicoPLEX WGA Kit uses a single-tube protocol developed specifically for the reproducible amplification of genomic DNA (gDNA) starting from 1–10 cells or equivalent picogram quantities of isolated gDNA. Cell lysis and whole-genome preamplification are followed by amplification with very low background to yield over 2 µg of product in under 3 hours. This is the kit of choice for assays that need the most accurate and reproducible measurement of CNV. This product contains reagents for 50 reactions.
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