Eppendorf BioSpectrometer® Fluorescence - EP6137000030

EP6137000030

The Eppendorf BioSpectrometers are spectrophotometers that combine easy handling with highly reproducible analysis. A variety of cuvettes expands the options even further from microvolume to macrovolume measurements.

Brand: Eppendorf

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Product CodeProduct	Product Description	Price (Excl gst)	Qty	Add
EP6137000030 Eppendorf BioSpectrometer® Fluorescence	Eppendorf BioSpectrometer® Fluorescence	Login for price

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The Eppendorf BioSpectrometer fluorescence is an expansion of the Eppendorf BioSpectrometer basic and features an integrated fluorescence module. Measurements can be flexibly executed in the UV/Vis or fluorescence range. The Eppendorf BioSpectrometer fluorescence can be used to increase the measuring range for biomolecules (nucleic acids and proteins) by a factor of 1,000, compared to UV measurements. What's more, fluorescence dyes allow a more accurate and reliable quantification due to the fact that these dyes bind specifically to the target biomolecule. UV/Vis spectroscopy and fluorescence are complementary methods. The Eppendorf BioSpectrometer fluorescence is the first spectrophotometer on the market which combines both measuring methods in one small, compact device.

Features

All Eppendorf BioSpectrometer basic options
Preprogrammed and freely programmable fluorescence methods
Fluorescence measurement concentration of dsDNA: 1 pg/µL – 1,000 pg/µL
Fluorescence measurement concentration of BSA: 0.01 ng/µL – 10 ng/µL
Fluorescence excitation wavelength 470 nm, emission wavelengths 520 nm and 560 nm
Fluorescence intensity across a range of 0.5 nM to 2,000 nM fluorescein

Absorption measuring range	0 A – 3.0 A (260 nm)
BSA concentration range	0.01 ng/µL – 45.45 µg/µL
BSA concentration range (UV 280 nm) µCuvette G1.0	75 ng/µL – 45.45 µg/µL ±0.03 µg/µL at A = 0 ±0.076 µg/µL at A = 1 (0.5 %)
BSA concentration range (UV 280 nm) UVette 10 mm	7.5 ng/µL – 4.5 µg/µL ±0.003 µg/µL at A = 0 ±0.007 µg/µL at A = 1 (0.5 %)
BSA concentration range (UV 280 nm) UVette 2 mm	37.5 ng/µL – 22.5 µg/µL ±0.015 µg/µL at A = 0 ±0.038 µg/µL at A = 1 (0.5 %)
Beam receiver absorption	CMOS photodiode array
Beam receiver fluorescence	Photodiodes
Cuvette shaft	12.5 mm × 12.5 mm
Emission wavelength I fluorescence	520 nm, bandwidth: 15 nm
Emission wavelength II fluorescence	560 nm, bandwidth: 40 nm
Excitation wavelength fluorescence	470 nm, bandwidth: 25 nm
Fluorescence measuring range	0.5 nM – 2,000 nM fluorescein (emission wavelength 520 nm)
Light path height	8.5 mm
Light source absorption	Xenon flash lamp
Light source fluorescence	LED
Measuring principle absorption	Single-beam absorption spectrophotometer with reference beam
Measuring principle fluorescence	Confocal filter fluorometer with reference beam
Method memory	>100 method programs
Mode absorption	Spectrophotometer
Mode fluorescence	Fluorometer
Operator guidance language	Spanish, Italian, French, English, German
Power consumption	Approx. 15 W during operating step Approx. 5 W with dimmed display
Random error absorption	≤0.002 at A = 0 ≤0.005 (0.5 %) at A = 1
Random error fluorescence	±2 % at 1 nM fluorescein
Random error wavelength	±1 nm
Random temperature error	–
Smallest step size	1 nm
Spectral bandwidth	≤4 nm
Systematic error wavelength	≤0.5 nm
Systematic temperature error	–
Wavelength range	200 nm – 830 nm
Wavelength range absorption	Scan (nm): 200 – 830 at 1 nm increments
dsDNA concentration range	1.0 pg/µL – 1,500 ng/µL
dsDNA concentration range (UV 260 nm) UVette 10 mm	2.5 ng/µL – 150 ng/µL ±0.1 ng/µL at A = 0 ±0.25 ng/µL at A = 1 (0.5 %)
dsDNA concentration range (UV 260 nm) UVette 2 mm	12.5 ng/µL – 750 ng/µL ±0.5 ng/µL at A = 0 ±1.25 ng/µL at A = 1 (0.5 %)
dsDNA concentration range (UV 260 nm) µCuvette G1.0	25 ng/µL – 1,500 ng/µL ±1 ng/µL at A = 0 ±2.5 ng/µL at A = 1 (0.5 %)
Interfaces	› USB master: for USB stick and thermal printer DPU-S445 › USB slave for connecting to a PC › Serial interface RS-232: for thermal printer DPU-414 › Ethernet interface RJ45: For connecting to a network printer
Power supply	100 – 240 V, 50 – 60 Hz
Dimensions (W × D × H)	295 × 400 × 150 mm / 11.6 × 15.7 × 6 in
Weight w/o accessories	5.4 kg

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