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MACHEREY-NAGEL purification products contribute to the fight against SARS-CoV-2 pandemic.

 

Facing the current COVID19 outbreak molecular testing laboratories rely on workflows to isolate SARS-CoV-2 RNA from respiratory samples.
Multiple labs have included MACHEREY-NAGEL´s NucleoSpin and NucleoMag kits for isolation of viral RNA into their workflows, among them the two WHO reference laboratories, the Institute Pasteur Paris (France) and the National Institute of Health (Thailand).

 

We are supporting all labs by providing guidelines for sample preparation and nucleic acid extraction with MN kits in compliance with WHO and CDC recommendations. The recommendations include all our NucleoSpin and NucleoMag kits suitable for extraction of viral RNA.

 

MN protocol recommendations for respiratory samples (swabs, sputum, bronchoalveolar lavage).

 

Current WHO references:

 

Relevant Kits

 

NucleoSpin Virus

 

NucleoSpin 8 / 96 Virus

 

Nucleomag Virus

Purification of human or animal viral RNA and DNA from serum, plasma, swab and tissues homegenates. Highly sensitive for DNA and RNA viruses e.g. HIV, HPV, H5N1, HBV, etc   Isolation of viral RNA and DNA from cellfree biological fluids in either an 8-well strip or 96-well format.   Magnetic bead based isolation of viral RNA and DNA from cell-free biological fluids. This kit can be adapted for automation systems.

 

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NucleoSpin RNA Virus

 

Nucleomag Pathogen

 

Nucleomag Vet

Isolation of only viral RNA from serum, plasma or cell-free biological fluids. Suitable for downstream RT-PCR and enzymatic reactions. Proven for large variety of viruses e.g. influenza, HCV, HIV, Bird Flu Virus.   DNA and microbial DNA from whole blood, serum or plasma, feces, tissue or swabs. Can be adapted for manual or automated processing.   Magnetic-bead purification of viral nucleic acids and bacterial DNA from veterinary and human samples, e.g. whole blood, serum, plasma, tissue homogenates, ear notches and swabs. Process manually or via automate

 

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Nucleomag DNA/RNA Water

Monitoring coronavirus in sewage could help to estimate the total number of infections. Infectious diseases that are excreted in urine or faeces, such as SARS-CoV-2, can be tracked by analyzing wastewater. Worldwide many researchers started to use wastewater testing to be able to early on identify a potential reoccurrence of the virus in certain communities. Routine wastewater surveillance might be an efficient, non-invasive tool to detect new COVID-19 infections and might help to limit the health and economic damaged caused by COVID-19.

 

To support our customers with this new approach we summarized the most common methods used for sample concentration, including filter- and non filter based methods. Because of their superior PCR inhibitor removal we recommend to either use NucleoMag® DNA/RNA Water.

 

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