The Capturem IP & Co-IP Kit is designed for the simple and rapid capture of protein-protein complexes from whole cell extracts. The kit contains specially designed Protein A spin columns, using our novel Capturem technology for efficient capture of antibody-bound target protein complexes. The included buffer set provides ease of use and rapid isolation of immunoprecipitated protein complexes, allowing faster downstream analysis.
Overview
- Simple, ultra-fast workflow: incubate IP antibody with sample for 10 minutes then purify at room temperature in 5 minutes
- Complete solution: includes Capturem Protein A spin columns and immunoprecipitation buffers optimized for IP and Co-IP experiments
- High concentration: extremely small elution volumes yield highly concentrated complexes
- High quality: short residence times reduce the possibility of aggregation, complex dissociation, or loss of activity
- Versatility: compatible with a wide range of IP buffers and conditions
- Single-use: novel membrane technology assembled into disposable spin columns reduces the risk of contamination and carryover
More Information
Applications
- Immunoprecipitation
- Co-immunoprecipitation
- Investigation of protein complexes
- Antigen screening for antibody discovery and engineering workflows
Isotype affinity of Protein A and Protein G
Species |
Antibody subtype |
Protein A |
Protein G |
Protein A/G |
Human |
Total IgG
IgG1
IgG2
IgG3
IgG4
IgM
IgD
IgA1
IgA2
Fab
scFv
|
+++
+++
+++
+
+++
+
−
+
+
+
+
|
+++
+++
+++
+++
+++
−
−
−
−
+
−
|
+++
+++
+++
+++
+++
+
−
+
+
+
+
|
Mouse |
Total IgG
IgM
IgG1
IgG2a
IgG2b
IgG3
|
+++
−
+
+++
+++
+++
|
+++
−
++
+++
+++
+++
|
+++
−
++
+++
+++
+++
|
Rat |
Total IgG
IgG1
IgG2a
IgG2b
IgG2c
|
+
+
−
−
+++
|
++
++
+++
+
+++
|
++
++
+++
+
+++
|
Rabbit |
Total IgG |
+++ |
+++ |
+++ |
Key: +++ strong binding; ++ medium binding; + weak binding; − no binding