Capturem Protein G Miniprep consists of single-use disposable columns for simple, rapid purification of antibodies that yield 50–100 μg of purified antibody per column, depending upon the sample type, species, and antibody isotype. These miniprep columns are suitable for purification of antibodies from animal sera, ascites fluid, cell culture media, and other sources.
Capturem Protein G 96 is a single-use disposable 96-well plate for simple, rapid purification of antibodies that yields up to 60 μg of purified antibody per well, depending upon species and antibody isotype. Capturem Protein G 96 is suitable for purification of antibodies from animal sera, ascites fluid, cell culture media and other sources.
Capturem Protein G Maxiprep columns are single-use disposable columns for simple, rapid purification of antibodies that yields up to 1–2 mg of purified antibody per column, depending upon antibody isotype and species. This maxiprep column is suitable for purification of antibodies from animal sera, ascites fluid, cell culture media and other sources.
Overview
- Fast and convenient: room-temperature protocol, complete in under 15 minutes, without incubation
- High purity: small column bed volumes trap fewer contaminants and allow large-volume washes
- High quality: short residence times reduce the possibility of aggregation or loss of activity
- Flexible: purify a wide range of antibodies
Note: Capturem Protein G 24-Well Plate - HC is optimized to give you an increased yield compared to our previous version.
More Information
Applications
- Antibody purification
- Immunoprecipitation
Approximate yields for Capturem Protein G formats
| Format |
Yield* |
Purification time
|
| Miniprep |
60 µg |
5 min |
| 96-well plate |
60 µg |
15 min |
| 24-well plate - HC |
600 µg** |
15 min |
| Maxiprep |
1.2 mg |
15 min |
*Yields vary depending upon the sample type, species, and antibody isotype.
**This yield was obtained when spiking 1 mg of mouse IgG1 in HEK293 cell supernatant.
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