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TaKaRa Taq™ DNA Polymerase Hot Start Version

These hot start versions of TaKaRa Taq DNA Polymerase contain a mixture of Taq polymerase and a monoclonal antibody to Taq polymerase. The antibody binds Taq polymerase, thereby preventing DNA synthesis. During the initial DNA denaturation step (reaction temperature ~94°C), the antibody is denatured, releasing the polymerase and allowing DNA synthesis to proceed. The use of a hot start PCR enzyme prevents nonspecific amplification due to mispriming and/or formation of primer dimers during PCR assembly.

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Product CodeProduct Product Description Price (Excl gst) Qty Add
TKR007B TaKaRa Taq™ DNA Polymerase Hot Start Version, 1,000 Units TaKaRa Taq™ DNA Polymerase Hot Start Version, 1,000 Units Login for price  
TKR007A TaKaRa Taq™ DNA Polymerase Hot Start Version, 250 Units TaKaRa Taq™ DNA Polymerase Hot Start Version, 250 Units Login for price  

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Features

  • All hot start Takara Taq versions contain an anti-Taq polymerase monoclonal antibody that improves specificity, reduces background, and allows room temperature assembly
  • TaKaRa Taq DNA Polymerase Hot Start Version: enzyme, buffer, and dNTPs are supplied as separate components.
  • Premix Taq DNA Polymerase Hot Start Version: 2X premix containing Takara Taq HS enzyme, dNTPs, and an optimized reaction buffer.
  • TaKaRa Taq HS Perfect Mix: 2X premix optimized for fast reactions; compatible with a 20 sec./kb extension step.