Features
- Indexed adapters compatible with Illumina® platforms for multiplexing libraries
- Up to 48 unique adapters available
- Considerably reduce your per-sample sequencing cost by multiplexing libraries
- Increase your sequencing scale by pooling 100s of samples on a single flow cell
- Validated on Illumina® next-generation sequencing platforms
- Compatible with the NEXTFLEX® Rapid RNA-Seq Kit and the NEXTFLEX® Rapid Directional RNA-Seq Kit
- Not compatible with the NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0
Added Flexibility to Library Preparation
The NEXTFLEX® RNA-Seq Barcodes are indexed adapters that are designed for use with the NEXTFLEX® Rapid RNA-Seq Kit and the NEXTFLEX® Rapid Directional RNA-Seq Kit. These barcodes are not recommended for use with the new NEXTFLEX® Rapid Directional RNA-seq kit 2.0.
These indexes are composed of a 6 nt error-correcting sequence, offering an improved multiplex workflow and adding flexibility to library prep. The ability to pool samples in an efficient way significantly decreases hands on time while providing robust data quality. Their automation-friendly format enables multiplexing of up to 48 samples. The NEXTFLEX® RNA-Seq Barcodes are available in sets of 6, 12, 24 and 48 unique adapters. We also offer the NEXTFLEX-96™ RNA-Seq Barcode Kits, which contain 96 – 8 nt index barcodes in a 96-well format (cat # NOVA-512915) .
KIT CONTENTS
- NEXTFLEX® RNA-Seq Barcode Adapter (0.6 µM)
- NEXTFLEX® Primer Mix (12.5 µM)
The NEXTFLEX® RNA-Seq Barcodes Kits contain 6, 12, 24 or 48 unique Illumina-compatible adapters, enabling the user to multiplex up to 48 samples per flow cell lane. These kits ship on dry ice.