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  4. Molecular
  5. Next Gen Sequencing Kits
  6. NEXTFLEX® Poly(A) Beads 2.0

NEXTFLEX® Poly(A) Beads 2.0

The NEXTFLEX® Poly(A) Beads 2.0 kit provides improved mRNA yields, lower rRNA contamination, and a more simplified protocol. The kit offers a convenient method for the purification of pure, intact mRNA upstream of RNA-Seq library prep. Poly(A)-tailed mRNA is isolated via magnetic beads conjugated to oligo(dT), and separation using a magnetic stand allows for high mRNA recovery. The intact mRNA is eluted in small volumes, thereby eliminating the need for precipitation. These beads have been validated with the new and improved NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0.

Brand: Revvity

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Product CodeProduct Product Description Price (Excl gst) Qty Add
PENOVA-512991 NEXTFLEX® Poly(A) Beads 2.0, 8rxns NEXTFLEX® Poly(A) Beads 2.0, 8rxns Login for price  
PENOVA-512992 NEXTFLEX® Poly(A) Beads 2.0, 48 rxns NEXTFLEX® Poly(A) Beads 2.0, 48 rxns Login for price  
PENOVA-512993 NEXTFLEX® Poly(A) Beads 2.0, 96 rxns NEXTFLEX® Poly(A) Beads 2.0, 96 rxns Login for price  

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Features

  • 10 ng – 5 µg total starting RNA
  • Magnetic bead-based protocol
  • No organic solvents or precipitation step required
  • Validated using the NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0
  • Magnetic bead-based protocol for purification of pure, intact mRNA upstream of RNA-Seq library prep (no organic solvents or precipitation step required)
  • Optimized for use with 10 ng – 5 µg of total RNA as starting material
  • Validated using the NEXTFLEX® Rapid Directional RNA-Seq Kit 2.0 and automated on the Sciclone® G3 NGSx and Zephyr® G3 NGS workstations

NEXTFLEX® POLY(A) BEADS KIT 2.0 CONTENTS

  • NEXTFLEX® Poly(A) Beads 2.0
  • NEXTFLEX® Poly(A) Elution Buffer 2.0
  • NEXTFLEX® Poly(A) Binding Buffer 2.0
  • NEXTFLEX® Poly(A) Washing Buffer 2.0

REQUIRED MATERIALS NOT PROVIDED

  • 10 ng - 5 μg total RNA
  • DynaMag™-2 Magnet (Life Technologies Cat # 123-21D) / or / similar
  • Water bath / Heat blocks
  • Tube Rotator-unit (VWR Cat # 13916-822) / or / similar
  • 2, 10, 20, 200 and 1000 μL pipettes
  • RNase-free pipette tips
  • Nuclease-free test tubes and microcentrifuge tubes
  • Microcentrifuge
  • Vortex

The NEXTFLEX® Poly(A) Beads 2.0 kit contains enough material to extract mRNA from 8, 48 or 96 total RNA samples for subsequent RNA-seq library prep or other applications. The kit ships with a minimum viable shelf life of at least six months. All components can be safely stored at 4°C.