Enjoy FREE SHIPPING on all FreeStyle Libre 2 purchases until 31 May 2024. Simply enter promo code FREESHIP at checkout and click ‘SUBMIT CODE’ to apply!

Pharmac proposes to commence FreeStyle Libre 2 subsidy for people living with type 1 diabetes! Read more

Order pick-ups are not available

X
Menu
Cart
X

Search Website

QUICK ORDER PRODUCTS

If you know the product code enter it here  

  1. Home
  2. Laboratory
  3. Molecular and Microbiology Reagents
  4. Molecular
  5. Gene Function
  6. Guide-it™ CRISPR/Cas9 System (Green or Red)

Guide-it™ CRISPR/Cas9 System (Green or Red)

The Guide-it CRISPR/Cas9 Systems are kits for the cloning and expression of target single guide RNAs (sgRNAs) for mammalian genome editing using CRISPR/Cas9 technology. The vector in this system simultaneously expresses Cas9 nuclease, a target-specific sgRNA, and an exceptionally bright fluorescent protein for monitoring transfection efficiency and/or for further enriching/isolating transfected cells by flow cytometry (ZsGreen1 and tdTomato versions are available).

Brand: Takara

Learn more

Select your options below
Enquire about this product
Product CodeProduct Product Description Price (Excl gst) Qty Add
CLT632601 Guide-it™ CRISPR/Cas9 System (Green) Guide-it™ CRISPR/Cas9 System (Green) Login for price  
CLT632602 Guide-it™ CRISPR/Cas9 System (Red) Guide-it™ CRISPR/Cas9 System (Red) Login for price  

Go to checkout

X

Stock Request

Submit your details.
You are enquiring about Guide-it™ CRISPR/Cas9 System (Green or Red)

Pricing Request Starts Here

Submit your details to receive a quote.
You are enquiring about Guide-it™ CRISPR/Cas9 System (Green or Red)

Related products

AAVpro® CRISPR/Cas9 Helper Free System (AAV2)

Product Code: CLT632608

View Product +

Guide-it™ Cas9 Polyclonal Antibody

Product Code: CLT632607

View Product +

APC/Fireª 750 anti-mouse CD25

Product Code: BIO102054

View Product +

The kit includes Stellar competent cells to ensure high efficiency transformation.

Features

  • A single vector expresses your target-specific sgRNA from the human U6 promoter, and Cas9 nuclease and a bright fluorescent marker (either ZsGreen1 or tdTomato) are expressed from a bidirectional CMV promoter
  • tdTomato and ZsGreen1 are exceptionally bright fluorescent proteins, 6x and 2.5x brighter than EGFP, respectively; these markers can be used to monitor transfection efficiency and/or to enrich/isolate transfected cells by flow cytometry
  • Simple plasmid construction—design oligos specific to your target sequence, and clone into the pre-linearized plasmid using the included ligation reagents and high-efficiency competent cells
  • Includes sufficient reagents for construction of 10 different target (sgRNA) expression plasmids
Components
Guide-it CRISPR/Cas9 System (Green) (Cat. No. 632601)
  • pGuide-it-ZsGreen1 Vector (Linear)
  • Guide-it Ligation Components
  • Stellar Competent Cells
Guide-it CRISPR/Cas9 System (Red) (Cat. No. 632602)
  • pGuide-it-tdTomato Vector (Linear)
  • Guide-it Ligation Components
  • Stellar Competent Cells