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BIO446004
The BioLegend ELISA MAX™ Deluxe Set is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA). The human PlGF capture antibody is first coated on a 96-well plate. Standards and samples are added to the wells and PlGFbinds to the immobilized capture antibody. Next, biotinylated anti-human PlGF detection antibody is added, producing an antibody-antigen-antibody “sandwich”. Avidin horseradish peroxidase is subsequently added, followed by TMB Substrate Solution, producing a blue color in proportion to the concentration of PlGF present in the sample. Finally, the Stop Solution changes the reaction color from blue to yellow, and the microwell absorbance is read at 450 nm with a microplate reader.
Brand: Biolegend
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