Lenti-X Expression System 

Lenti-X Concentrator provides a fast, simple, and highly efficient method for concentrating any lentiviral stock. In the simple protocol, you just mix your lentiviral supernatant with the lentivirus concentration reagent, incubate for a short period, and spin the mixture in a standard centrifuge.

100-fold increase in titer

You will increase your lentiviral titer by up to 100-fold in ~1 hr, and obtain excellent recoveries with no ultracentrifugation. Lenti-X Concentrator works for all lentiviral supernatants, including those made from any Takara Bio-brand Lenti-X system, and the procedure can be scaled up or down to best suit your needs.

Far simpler than ultracentrifugation

Compared to ultracentrifugation, the Lenti-X Concentrator is more flexible in terms of scale, is easier to use, and requires no specialized equipment.

Scalable for supernatants of any volume, any titer

The Lenti-X Concentrator reagent is a 4X concentrate so that can be added to any volume of supernatant containing any amount of virus or any starting titer. In our hands, scalability has been successful for supernatant volumes up to 250 ml.

Overview

• Simple mix-and-spin protocol
• Hassle-free and easily scaled up for large volumes
• No ultracentrifugation required
• 100X concentration
• 90% yield
• Lenti-X Concentrator can also concentrate MMLV and MSCV retrovirus supernatants utilizing the longer incubation times