Applications include
- Staining and enumeration of CD4+ CD25+ CD127dim/– regulatory T cells in PBMCs, apheresis products, umbilical cord blood, or whole blood
- Analysis of Treg cells separated using MACS® Technology
Background information
The kit combines monoclonal antibodies against the standard Treg cell markers CD4, CD25, and CD127 as well as the leukocyte marker CD45 at optimized titers in one vial. The combination of CD4, CD25, and CD127 staining is the current standard method for the identification of live Treg cells. Several publications have reported a strong correlation between the CD25+ CD127dim/– phenotype, expression of FoxP3, and suppressive function in CD4+ cells.